Construction of a Minigenome Rescue System for Measles Virus, AIK-c Strain

construction of a minigenome rescue system for measles virus, aik-c strain

background:in the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded rna viruses from cdna or viral minigenomes. this technique has been applied to study different steps in virus replication and virus-host interactions. reverse genetics could also be implemented for design of new vaccines. the t7 rna polymerase activity as well as virus (nucleocapsi...

Recombinant Measles AIK-C Vaccine Strain Expressing the prM-E Antigen of Japanese Encephalitis Virus

An inactivated Japanese encephalitis virus (JEV) vaccine, which induces neutralizing antibodies, has been used for many years in Japan. In the present study, the JEV prM-E protein gene was cloned, inserted at the P/M junction of measles AIK-C cDNA, and an infectious virus was recovered. The JEV E protein was expressed in B95a cells infected with the recombinant virus. Cotton rats were inoculate...

Sequence Variation in Measles Virus F Gene of AIK-C Vaccines Strain after Passage in Vero Cells

Stability Study of Measles AIK-C Strain, Mumps RS-12 Strain and Rubella Takahashi Strain in MMR Vaccine

Stability studies play a critical role in assuring product quality at all points in the vaccine life cycle and havea major impact on the success of immunization programs worldwide. The purpose of stability study isdetermination of the vaccine quality under the variety of environmental factors and to establish a re-testperiod or a shelf life and recommended storage conditions. In this study thre...

Leucine at position 278 of the AIK-C measles virus vaccine strain fusion protein is responsible for reduced syncytium formation.

The live measles virus (MV) vaccine strain AIK-C was attenuated from the wild-type strain Edmonston by plaque purification at 33 degrees C. Strain AIK-C grew well at 33 degrees C with a mixture of small-and medium-sized plaques in Vero cells, but did not grow well at 40 degrees C. To investigate fusion inducibility, expression plasmids for the fusion (F) and haemagglutinin (H) protein regions o...

Buthionine Sulfoximine Inhibits Cytopathic Effects and Apoptosis Induced by Infection with AIK-HDC Strain of Measles Virus

Measles virus (MV) is a highly contagious agent which causes a major health problem in developing countries. We studied the effect of buthionine sulfoximine (BSO) on the replication of an AIK-HDC strain of MV and its induced apoptosis in Vero cell lines. Methods: In this study, toxicity of BSO on Vero cells was investigated first, resulted in determination of sub-lethal or non-toxic concentrati...